COMMON TYPES OF MICROBES
Difference between Gram Positive and Gram Negative Shapes of bacteria and cases What designs have you discovered? GRAM STAIN: The process! You must work on your lab notes. LECTURE a couple of
Developed by a Danish named Christian Gram in 1884 Stain a heat-fixed smear with a dye like amazingly violet and fix with Iodine After that wash with ethanol or perhaps acetone Finally counterstain using a dye of different colour including safranin (what is it is colour? You may have used it prior to! )
a couple of
GRAM POSITIVE CELL WALL
a thick, homogenous sheath of peptidoglycan 20-80 nm thicker tightly destined acidic polysaccharides, including teichoic acid and lipoteichoic acid solution cell membrane layer
Retain crystal violet and stain magenta
GRAM POSITIVE WALL
GRAM NEGATIVE CELL WALL
on the lookout for
an outer membrane containing lipopolysaccharide (LPS) slender shell of peptidoglycan periplasmic space internal membrane
Drop crystal purple and discolor red coming from safranin counterstain
GRAM BAD CELL WALL STRUCTURE
Resting, heavy cells created by some G+ genera: Clostridium, Bacillus & Sporosarcina Include a 2-phase life circuit вЂ“ vegetative cell & an endospore
sporulation -formation of endospores germination- go back to vegetative progress hardiest of all life forms withstand extremes in high temperature, drying, very cold, radiation & chemicals not only a means of reproduction
resistance linked to high numbers of calcium & dipicolinic acid dehydrated, metabolically inactive heavy coat durability verges in immortality twenty-five, 250 mil years. pressurized steam for 120oC for 20-30 minutes will eliminate.
Producing of tradition medium 2 . Recall the investigations in Culturing Performance of Soaps and Alcohols What kind of culture medium did you use? Milks What did you are doing to investigate the result on " temperatureвЂќ treatment to milk? 1 .
PHYSICAL AND CHEMICAL
PHYSICAL AND CHEMICAL SUBSTANCE
Sanitation Disinfection Sanitization Antiseptic Pasteurization
Description Universal/ Standard Precautions Make use of disinfectants Vincent's Lecture
Prof Maxwell's notes and this lecture! Explanations What are the regular infectious conditions by prions? Any treatment options? Epidemiology, aetiology, вЂ¦вЂ¦.
Prof Maxwell's notes What is the disease that is brought on by " tapewormвЂќ? Aetiology, epidemiology, sign and symptoms, pathogenesis, prevention and control
Methicillin-resistant Staphylococcus aureus (MRSA) infections Control of infection in health care adjustments: 1 . Control the water tank 2 . Interrupt the tranny 3. Prevent access of agent to new web host 4. Boost resistance of recent host
Key reservoir: individuals вЂў S. aureus can be normal botanica - Nares - Goitre - Skin - Axilla - Perineum
Patient-to-patient via transitive carriage in hands of Health Care Staff (HCW) HCW-to-patient through serious HCW company Via gadgets or environmental surface
Infection control procedures: Contact safety measures -Hand health -Protective barriers - Individual placement -- Environmental cleaning - Patient education -- Patient travel - Devoted patient care equipment Suitable use of antibiotics
CHAIN OF INFECTION
CHAIN OF INFECTION
We. Infection circuit is well defined A. Infective agent: pathogen such as a bacteria or virus N. Reservoir 1 ) Place where causative agent can live 2 . Prevalent reservoirs contain human body, pets or animals, environment, and fomites or objects contaminated with contagious material which has the pathogens
CHAIN OF INFECTION
C. Portal of exit 1 . Way for causative agent to escape from the water tank 2 . Pathogens can leave the body through urine, feces, saliva, blood, tears, mucous discharge,...